Histology Protocols

85,39 € 82,11 €
Disponible
ISBN
9781603273442
Edición
I
Autores
Hewitson, Tim D., Darby, Ian A.
Editorial
Springer
Fecha Publicacion
1 ene. 2010
Características
N/D
Table of contents

Histology protocols

eds Tim Hewitson & Ian Darby

Part A: Tissue processing

Fixation (immersion and perfusion fixation)

Embedding and Sectioning

Antigen retrieval (enzyme, pressure cooker and microwave pre-treatments)

Laser capture microscopy

Part B: Histochemistry

Immunohistochemistry for light microscopy

(Dab, alk phos, fluorescence)

Immunogold histochemistry for electron microscopy

In situ hybridisation

(Cells, tissues, EM)

In situ localization of apoptosis

(TUNEL, annexin V/propidium iodide)

Identification of tissue hypoxia in situ

Immunophenotyping of cell populations

Special stains for extracellular matrix

Lectin histochemistry

Part C: Imaging Techniques

Electron microscopy

Confocal microscopy

In vivo imaging of GFP-labelled cells

Quantitative image analysis

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