Current protocols essential laboratory techniques 2nd ed.

Description

The newest addition to the esteemed Current Protocols series, Current Protocols Essential Laboratory Techniques, 2e, equips novice researchers with essential skills and a solid understanding of key laboratory techniques required to conduct successful experiments, tackle challenges, and thrive as effective contributors within contemporary life science laboratories. This comprehensive guide covers foundational competencies such as measurement, reagent preparation, and utilization of basic instruments, while also delving into advanced methodologies like blotting, chromatography, and real-time PCR. As a practical reference manual, this book is invaluable for any life science researcher. Authored by a blend of renowned experts and distinguished faculty, Current Protocols Essential Laboratory Techniques, 2e serves as a fundamental resource for developing the skills needed to embark on a rewarding research career.

Table of Contents

  • Foreword
  • Preface
  • Contributors List
  • Conversion Factors
  • Flow Chart: Application of Techniques
  • Chapter 1: Volume/Weight Measurement
    • 1. Volume Measurement – Andrew Zanella and Tom Davis, Claremont Colleges
    • 2. Weight Measurement – Michael Guzy, Ohaus, Corp.
  • Chapter 2: Concentration Measurement
    • 1. Spectrophotometry – Rob Morris, Ocean Optics, Inc.
    • 2. Quantitation of DNA and RNA with Absorption and Fluorescence Spectroscopy – Sean R. Gallagher, UVP, LLC
  • Chapter 3: Reagent Preparation
    • 1. Theoretical Discussion – Deb Chakravarti, Keck Graduate Institute
    • 2. Practical Discussion – Deb Chakravarti, Keck Graduate Institute
    • 3. Recipes In-House
  • Chapter 4: Cell Culture Techniques
    • 1. Aseptic Technique – Thomas Bykowski and Brian Stevenson, University of Kentucky College of Medicine
    • 2. Culture of Escherichia coli and Related Bacteria – Sean P. Riley, Michael E. Woodman, and Brian Stevenson, University of Kentucky College of Medicine
  • Chapter 5: Sample Preparation
    • 1. Centrifugation and Cell Fractionation In-House
    • 2. Purification and Concentration of Nucleic Acids – Dennis H. Dowhan, University of Queensland
  • Chapter 6: Chromatography
    • 1. Overview of Chromatography – Buddhadeb Mallik, Bulbul Chakravarti, and Deb N. Chakravarti, Keck Graduate Institute of Applied Life Sciences
    • 2. Column Chromatography – Deb Chakravarti, Keck Graduate Institute
  • Chapter 7: Electrophoresis
    • 1. Overview of Electrophoresis – Sean R. Gallagher, UVP, LLC
    • 2. Agarose Gel Electrophoresis – Jennifer A. Armstrong, The Claremont Colleges, and Joseph R. Schulz, Occidental College
    • 3. SDS-PAGE – Sean Gallagher, UVP, LLC
    • 4. Staining of Gels – Sean Gallagher, UVP, LLC
  • Chapter 8: Blotting
    • 1. Overview of Blotting – Maria Cristina Negritto, Pomona College, and Glenn M. Manthey, City of Hope, Beckman Research Institute
    • 2. Nucleic Acid Blotting: Southern and Northern – Laura L. Mays Hoopes, Pomona College
    • 3. Protein Blotting: Immunoblotting – Sean Gallagher, UVP, LLC
    • 4. Labeling DNA and Probe Preparation – Karl A. Haushalter, Harvey Mudd College
  • Chapter 9: Microscopy
    • 1. Conventional Light Microscopy – Eric S. Cole, St. Olaf College
    • 2. Immunofluorescence Microscopy – David J. Asai, Harvey Mudd College
  • Chapter 10: Enzymatic Reactions
    • 1. Working with Enzymes – David Skrincosky and Cindy Santangelo, Worthington Biochemical Corp.
    • 2. Overview of PCR – Christine D. Kuslich, Molecular Profiling Institute, Buena Chui, GE Healthcare, and Carl T. Yamashiro, Molecular Profiling Institute
    • 3. Real-Time PCR – Dean Fraga, College of Wooster, Tea Meulia, Ohio Agricultural Research and Development Center, Steven Fenster, Ashland University
  • Chapter 11: Bioinformatics
    • 1. Using NCBI Blast – Nicholas A. Stover, Bradley University, and Andre R.O. Cavalcanti, Pomona College
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